Composite

Part:BBa_K3245018

Designed by: Jianyi Hu   Group: iGEM19_Fudan   (2019-10-21)

Part Name luxpR-HS-EsfGFP-B0014

Description:

This composite part is used for measurement of the former mutated promoter. The promoter provides low leakage and a comparatively low expression level. An improved reporter protein EsfGFP ( BBa_K3245008 ) with super fluorescence can make up for the insufficiency.

Usage:

Transfer with high copy control plasmid containing luxI-luxR part ( BBa_K3245002 ) to test real time quorum sensing behavior and collect data such as OD600 and fluorescence. Transfer with plasmid containing luxR part ( BBa_C0062, etc. ) and start measuring with artificial AHL induction. It’s more suitable when accurate mechanism or dynamics research is needed.

Characterization:

Please refer to https://parts.igem.org/Part:BBa_K2558001


This composite part is used for measurement of the former promoter.

Tsinghua 2018 reported that lux pR-HS solved the leakage problem of luxpR. We linked it with our reporter EsfGFP to test its behavior in the QS system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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